|
| Status |
Public on Jul 06, 2015 |
| Title |
K. lactis Ste12-myc ChIP-Seq in pheromone-responding a cells |
| Organism |
Kluyveromyces lactis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The purpose of this experiment was to determine the genes directly regulated by Ste12 in K. lactis. The experiment was performed in a cells to determine if the a-specific genes were bound by Ste12. Ste12 was tagged with c-myc and was immunoprecipitated with a c-myc antibody. Cells were starved in SD media lacking phosphate for 2 hours, then treated with 10µg/mL K. lactis alpha factor for 2 hours before harvesting. A control untagged strain was processed in parallel. The experiment was repeated 3 times, and sequenced on an Illumina HiSeq 2500.
|
| |
|
| Overall design |
K. lactis Ste12-myc vs. untagged control under pheromone response condition with 3 technical replicates done on different days
|
| |
|
| Contributor(s) |
Sorrells TR |
| Citation(s) |
26153861 |
| |
| Submission date |
Feb 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Trevor Sorrells |
| E-mail(s) |
trevorsorrells@gmail.com
|
| Organization name |
University of California, San Francisco
|
| Department |
Biochemistry and Biophysics
|
| Lab |
Johnson
|
| Street address |
600 16th St N374
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19759 |
Illumina HiSeq 2500 (Kluyveromyces lactis) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA275069 |
| SRA |
SRP053419 |
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