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Series GSE65834 Query DataSets for GSE65834
Status Public on Jan 09, 2016
Title Transcriptional profiling of HBV-naïve subjects before vaccination against Hepatitis A/B viruses, Diphtheria/Tetanus toxoids and Cholera.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Mechanisms of poor responses to vaccines remain unknown. Hepatitis B virus-naïve elderly subjects received three vaccines, including a vaccine against hepatitis B virus (HBV). Pre-vaccination high dimensional analyses of blood using transcriptional profiling and flow cytometry revealed that subjects having increased memory B cell frequencies and higher expression of genes downstream of B cell receptor signaling responded more strongly to the HBV vaccine whereas subjects having higher expression of inflammatory related genes and greater numbers of activated innate immune cells showed a weaker response to this vaccine. The heme-induced response was associated with the poor response to the hepatitis B vaccine. Transcriptional profiling and flow cytometry results were validated in a distinct set of elderly subjects with accuracy greater than 60%. Our study is the first that identifies baseline predictors of responses to vaccines in a population of subjects known to be highly susceptible to infections.
 
Overall design One hundred and seventy four (174) generally healthy, Hepatitis B virus (HBV) naïve, adult residents of Québec were vaccinated with two doses of Twinrix® (HBsAg and Hepatitis A virus - Glaxo Smith-Kline), generic Tetanus-diphtheria booster (tetanus and diphtheria - Sanofi-Pasteur), and Dukoral (recombinant cholera toxin B subunit and whole killed Vibrios - Sanofi Pasteur) according to the respective product labels. Blood samples were taken immediately prior to vaccination. Blood samples were conserved in PAXgene tubes. RNA was extracted and hybridized to Affymetrix arrays. 5 technical replicates were included in the study.
 
Contributor(s) Fourati S, Cristescu R, Loboda A, Talla A, Filali A, Railkar R, Wang I, Carayannopoulos LN, Sékaly R
Citation(s) 26742691
Submission date Feb 10, 2015
Last update date May 07, 2018
Contact name Slim Fourati
E-mail(s) slim.fourati@northwestern.edu
Organization name Northwestern University
Department Medicine
Lab Fourati
Street address 300 E Superior St
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray [HuRSTA_2a520709.CDF]
Samples (169)
GSM1607185 pre-vaccination blood sample from donor 1033
GSM1607186 pre-vaccination blood sample from donor 1013
GSM1607187 pre-vaccination blood sample from donor 2007
Relations
BioProject PRJNA275222

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65834_RAW.tar 744.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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