|
| Status |
Public on Apr 22, 2016 |
| Title |
PDGFRα+ cells in ESC cultures represent the in vitro equivalent of the pre-implantation primitive endoderm precursors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mouse Embryonic Stem Cells (ESCs) express PDGFRα heterogeneously, fluctuating between a PDGFRα+ (PrE-primed) and a Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third subpopulation, expressing epiblast and PrE determinants.
|
| |
|
| Overall design |
Three different subpopulatiosn were sorted based on PECAM1/PDGFRa expression and analyzed by NGS
|
| |
|
| Contributor(s) |
Lo Nigro A, Verfaillie CM |
| Citation(s) |
28089671 |
| |
| Submission date |
Feb 12, 2015 |
| Last update date |
Sep 27, 2019 |
| Contact name |
Antonio Lo Nigro |
| E-mail(s) |
antoniolonigro83@gmail.com
|
| Organization name |
Ismett
|
| Street address |
Via tricomi 5
|
| City |
Palermo |
| ZIP/Postal code |
90134 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA275302 |
| SRA |
SRP054972 |
Less...
More...