|
| Status |
Public on Apr 21, 2015 |
| Title |
Early postnatal expression data from mouse skeletal muscle stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Satellite cells are the primary source of stem cells for skeletal muscle growth and regeneration. Since adult stem cell maintenance involves a fine balance between intrinsic and extrinsic mechanisms, we performed genome-wide chronological expression profiling to identify the transcriptomic changes involved during early postnatal growth till acquisition of satellite cell quiescence.
|
| |
|
| Overall design |
Muscle samples were isolated from the trunk of Pax3GFP/+ mice. After digestion, GFP cells were purified via FACS and process for RNA extraction and hybridization on Affymetrix microarrays (Affymetrix Mouse Genome 430 2.0 Arrays).
The different ages selected for sample isolation were P1-P12-P28 (P, postnatal days), covering proliferant and quiescent satellite cells. The three stages were done in triplicate for P28 and 4 times for P1-P12, so 11 samples included in the microarray.
|
| |
|
| Contributor(s) |
Alonso-Martin S, Rochat A, de Reyniès A, Relaix F |
| Citation(s) |
25742797 |
| |
| Submission date |
Feb 13, 2015 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Sonia Alonso-Martin |
| E-mail(s) |
alonsomartin.s@gmail.com
|
| Phone |
+33684321566
|
| Organization name |
Myology Research Center U974-INSERM - UPMC-Paris VI - Institut de Myologie
|
| Department |
Development and stem cells
|
| Lab |
Team 8 - Relaix
|
| Street address |
105 bd de l'Hôpital
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA275424 |
Less...
More...