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Series GSE661 Query DataSets for GSE661
Status Public on Mar 05, 2004
Title ABA or GA calli treatment
Organism Oryza sativa
Experiment type Expression profiling by array
Summary The callus used for total RNA extraction was derived from the scutellum of the japonica rice variety Nipponbare and cultivated in Murashige and Skoog medium* containing 10 microM 2,4 dichlorophenoxyacetic acid. Such callus maintains the ability to develop roots and leaves. After the calli had been cultured in the medium for 30 d, they were transferred to a medium containing either ABA or GA (Gibberellin) plant hormones and cultured for 3 d. The concentration of the plant hormone was adjusted to 50 microM. After culturing, we used an RNeasy Plant Mini Kit (QIAGEN, Tokyo, Japan) to extract total RNA from the hormone-treated calli and from the controls. Messenger RNA (mRNA) was isolated with an Oligotex-dt30 (Super) mRNA purification kit (TaKaRa, Shiga, Japan). Purified mRNA was amplified, labeled, and hybridized to the NIAS RICE 22K oligonucleotide array ver1 according to the manufacturer.
Keywords: other
 
 
Contributor(s) Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Shimbo K, Kikuchi S
Citation(s) 14982972
Submission date Sep 10, 2003
Last update date Mar 02, 2012
Contact name Junshi Yazaki
E-mail(s) yaz@nias.affrc.go.jp
Phone 81-29-838-7007
Fax 81-29-838-7007
URL http://cdna01.dna.affrc.go.jp/RMOS/index.html
Organization name National Institute of Agrobiological Sciences
Department Molecular Genetics
Lab Gene Expression
Street address 2-1-2 Kan-nondai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8602
Country Japan
 
Platforms (1)
GPL477 RICE oligo microarray ver1
Samples (8)
GSM9853 50uM ABA_24
GSM9854 50uM ABA_20
GSM9855 50uM ABA_19
Relations
BioProject PRJNA87595

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Supplementary data files not provided

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