|
| Status |
Public on Feb 21, 2015 |
| Title |
The Effects of Nanog HD Mutants on Mouse Embryonic Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We examined global gene expression patterns of mouse embryonic stem cells overexpressing EGFP, Nanog wild-type, or Nanog L122A mutants in normal, "-LIF" or "+RA" culture conditions by RNA-seq experiments. In “+RA” culture conditions, the expression patterns of the RA-responsive genes were slightly different in WT transfectants and more different in L122A transfectants compared with EGFP transfectants. These results suggested that L122A transfectants showed partial resistant activity against RA-induced differentiation, which was not found in WT mNANOG transfectants.
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| |
|
| Overall design |
Examination of 3 different transfectants from mouse embryonic stem cell, RF8 line, in 3 different culture conditions.
|
| |
|
| Contributor(s) |
Hayashi Y, Yamanaka S |
| Citation(s) |
25825768 |
| |
| Submission date |
Feb 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gladstone Bioinformatics |
| Organization name |
Gladstone Institutes
|
| Department |
Data Science and Biotechnology
|
| Lab |
Bioinformatics Core
|
| Street address |
1650 Owens St
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA275930 |
| SRA |
SRP055200 |
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