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Series GSE66223 Query DataSets for GSE66223
Status Public on Jul 01, 2015
Title Genome-wide profiling of H3K56ac in Drosophila melanogaster Kc167 cell lines with Lz overexpression
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The conserved Notch pathway functions in diverse developmental and disease-related processes, requiring mechanisms to ensure appropriate target-selection and gene activation in each context. To investigate, we partitioned Drosophila chromatin into different states, based on histone modifications, establishing the preferred chromatin conditions for binding of CSL, the Notch pathway transcription factor and showing that a co-operating factor, Lozenge/Runx, can help establish these conditions. While most histone modifications were unchanged by CSL binding or Notch activation, rapid changes in H3K56 acetylation occurred at Notch regulated-enhancers. This modification extended over large regions, required the histone acetyl-transferase CBP and was independent of transcription. Such rapid changes in H3K56 acetylation are a conserved indicator of enhancer activation, also occurring at mammalian Notch-regulated Hey1 and at Drosophila ecdysone-regulated genes. This core histone modification may therefore underpin the changes in chromatin accessibility needed to promote transcription following signaling activation.
 
Overall design H3K56ac profile of control cells (KP) and of Lz overexpression cells (KL). In total 3 samples, H3K56ac ChIP in KP cells and 2 replicates of H3K56ac ChIP in KL cells.
 
Contributor(s) Li J, Fischer BE, Bray SJ
Citation(s) 26069324
Submission date Feb 23, 2015
Last update date Jul 08, 2015
Contact name Sarah Bray
E-mail(s) sjb32@cam.ac.uk
Organization name University of Cambridge
Department PDN
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3DY
Country United Kingdom
 
Platforms (1)
GPL6629 [DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array
Samples (3)
GSM1617424 KL H3K56ac rep1
GSM1617425 KL H3K56ac rep2
GSM1617426 KP H3K56ac
This SubSeries is part of SuperSeries:
GSE66227 Chromatin signatures at Notch regulated enhancers reveal large-scale changes in H3K56ac upon activation
Relations
BioProject PRJNA276137

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66223_RAW.tar 166.6 Mb (http)(custom) TAR (of BEDGRAPH, CEL)
Processed data provided as supplementary file

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