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Status |
Public on Jul 01, 2015 |
Title |
Genome-wide profiling of H3K56ac in Drosophila melanogaster Kc167 cell lines with Lz overexpression |
Organism |
Drosophila melanogaster |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
The conserved Notch pathway functions in diverse developmental and disease-related processes, requiring mechanisms to ensure appropriate target-selection and gene activation in each context. To investigate, we partitioned Drosophila chromatin into different states, based on histone modifications, establishing the preferred chromatin conditions for binding of CSL, the Notch pathway transcription factor and showing that a co-operating factor, Lozenge/Runx, can help establish these conditions. While most histone modifications were unchanged by CSL binding or Notch activation, rapid changes in H3K56 acetylation occurred at Notch regulated-enhancers. This modification extended over large regions, required the histone acetyl-transferase CBP and was independent of transcription. Such rapid changes in H3K56 acetylation are a conserved indicator of enhancer activation, also occurring at mammalian Notch-regulated Hey1 and at Drosophila ecdysone-regulated genes. This core histone modification may therefore underpin the changes in chromatin accessibility needed to promote transcription following signaling activation.
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Overall design |
H3K56ac profile of control cells (KP) and of Lz overexpression cells (KL). In total 3 samples, H3K56ac ChIP in KP cells and 2 replicates of H3K56ac ChIP in KL cells.
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Contributor(s) |
Li J, Fischer BE, Bray SJ |
Citation(s) |
26069324 |
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Submission date |
Feb 23, 2015 |
Last update date |
Jul 08, 2015 |
Contact name |
Sarah Bray |
E-mail(s) |
sjb32@cam.ac.uk
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Organization name |
University of Cambridge
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Department |
PDN
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Street address |
Downing Street
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City |
Cambridge |
ZIP/Postal code |
CB2 3DY |
Country |
United Kingdom |
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Platforms (1) |
GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE66227 |
Chromatin signatures at Notch regulated enhancers reveal large-scale changes in H3K56ac upon activation |
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Relations |
BioProject |
PRJNA276137 |
Supplementary file |
Size |
Download |
File type/resource |
GSE66223_RAW.tar |
166.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH, CEL) |
Processed data provided as supplementary file |
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