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GEO help: Mouse over screen elements for information. |
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Status |
Public on Dec 01, 2007 |
Title |
Gene expression program of AM-14 B cells stimulated through the B cell receptor (BCR) and/or Toll-like receptors (TLR) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
We have previously shown that rheumatoid factors (RF) produced by Fas-deficient autoimmune-prone mice typically bind autologous IgG2a with remarkably low affinity. Nevertheless, B cells representative of this RF population proliferate vigorously in response IgG2a/chromatin immune complexes through a mechanism dependent on the sequential engagement of the BCR and Toll-like receptor 9 (TLR9). To more precisely address the role of both receptors in this response, we analyzed the signaling pathways activated in AM14 B cells stimulated with these complexes. We found that the BCR not only serves to direct the chromatin complex to an internal compartment where it can engage TLR9 but also transmits a suboptimal signal that in combination with the signals emanating from TLR9 leads to NF?B activation and proliferation. Importantly, engagement of both receptors leads to the upregulation of a group of gene products, not induced by the BCR or TLR9 alone, that include IL-2. These data indicate that autoreactive B cells, stimulated by a combination of BCR and TLR9 ligands, acquire functional properties that may contribute to the activation of additional cells involved in the autoimmune disease process. Keywords: cell stimulation
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Overall design |
15 Samples, 3 replicates for each treatment
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Citation(s) |
18025183 |
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Submission date |
Jan 08, 2007 |
Last update date |
Feb 11, 2019 |
Contact name |
Jason W Bauer |
E-mail(s) |
baue0271@umn.edu
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Phone |
612-625-5168
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Organization name |
University of Minnesota
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Street address |
420 Deleware St SE MMC108
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City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (15)
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GSM154247 |
AM14 B cells, anti IgM treated, rep1 |
GSM154248 |
AM14 B cells, anti IgM treated, rep2 |
GSM154249 |
AM14 B cells, anti IgM treated, rep3 |
GSM154250 |
AM14 B cells, CpG treated, rep1 |
GSM154251 |
AM14 B cells, CpG treated, rep2 |
GSM154252 |
AM14 B cells, CpG treated, rep3 |
GSM154253 |
AM14 B cells, PL2-3, rep1 |
GSM154254 |
AM14 B cells, PL2-3, rep2 |
GSM154255 |
AM14 B cells, PL2-3, rep3 |
GSM154256 |
AM14 B cells, anti-IgM+CpG, rep1 |
GSM154257 |
AM14 B cells, anti-IgM+CpG, rep2 |
GSM154258 |
AM14 B cells, anti-IgM+CpG, rep3 |
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Relations |
BioProject |
PRJNA98613 |
Supplementary file |
Size |
Download |
File type/resource |
GSE6674_RAW.tar |
90.0 Mb |
(http)(custom) |
TAR (of CEL) |
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