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| Status |
Public on Oct 15, 2015 |
| Title |
Gene expression changes during serum starvation-induced quiescence in human fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We report a comparison of gene expression profiling microarray datasets generated from proliferating and serum-starved quiescent human normal diploid fibroblasts. 4 biological replicates of proliferative and quiescent fibroblasts were subjected to 4 individual dye-swap experiments. Data from these arrays were used in a publication to compare reproducibility between microarrays and RNA-seq; RNA-seq (2 replicates of each proliferative and quiescent fibroblasts) are available through the GEO (accession #: GSE65145). Correlations between both the microarrays and the RNA-seq dataset showed only moderate concordance (r =0.42) in genes exhibiting a ≥5-fold change.
Examination of gene expression profiles from prolfierating and quiescent normal human fibroblasts using microarray technology
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| Overall design |
Analysis used proliferative expression as a baseline and quiescent samples were compared against this. Four independent arrays were used with dye swaps to make direct comparisions between quiescent and prolfierative gene expression levels.
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| Contributor(s) |
Eskiw C |
| Citation(s) |
26473061 |
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Brett Trost, Catherine A. Moir, Zoe E. Gillespie, Anthony Kusalik, Jennifer A. Mitchell, Christopher H. Eskiw. Concordance between RNA-sequencing data and DNA microarray data in transcriptome analysis of proliferative and quiescent fibroblasts. R Soc Open Sci. 30 Sept 2015 http://dx.doi.org/10.1098/rsos.150402
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| Submission date |
Mar 11, 2015 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Christopher H. Eskiw |
| E-mail(s) |
c.eskiw@usask.ca
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| Phone |
306-966-2454
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| Organization name |
University of Saskatchewan
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| Department |
Food and Bioproduct Science
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| Lab |
Eskiw Lab
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| Street address |
51 Campus Drive
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| City |
Saskatoon |
| State/province |
SK |
| ZIP/Postal code |
S7N 5A8 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA277961 |
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