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| Status |
Public on Apr 01, 2015 |
| Title |
Reprogramming of primary human Philadelphia chromosome-positive B cell acute lymphoblastic leukemia cells into nonleukemic macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
BCR–ABL1+ precursor B-cell acute lymphoblastic leukemia (BCR– ABL1+ B-ALL) is an aggressive hematopoietic neoplasm characterized by a block in differentiation due in part to the somatic loss of transcription factors required for B-cell development. We hypothesized that overcoming this differentiation block by forcing cells to reprogram to the myeloid lineage would reduce the leukemogenicity of these cells. We found that primary human BCR–ABL1+ B-ALL cells could be induced to reprogram into macrophage-like cells by exposure to myeloid differentiation-promoting cytokines in vitro or by transient expression of the myeloid transcription factor C/EBPα or PU.1. The resultant cells were clonally related to the primary leukemic blasts but resembled normal macrophages in appearance, immunophenotype, gene expression, and function. Most importantly, these macrophage-like cells were unable to establish disease in xenograft hosts, indicating that lineage reprogramming eliminates the leukemogenicity of BCR–ABL1+ B-ALL cells, and suggesting a previously unidentified therapeutic strategy for this disease. Finally, we determined that myeloid reprogramming may occur to some degree in human patients by identifying primary CD14+ monocytes/ macrophages in BCR–ABL1+ B-ALL patient samples that possess the BCR–ABL1+ translocation and clonally recombined VDJ regions.
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| Overall design |
We obtained the expression profiles of 5 human B-ALL samples using Afymmetrix U133A2 microarrays. Blasts were either analyzed without culture, or cultured in the presence of myeloid cytokines and sorted into CD14+ and CD19+ populations.
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| Contributor(s) |
McClellan JS, Dove C |
| Citation(s) |
25775523 |
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| Submission date |
Mar 11, 2015 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Christopher Dove |
| Phone |
6507368078
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| Organization name |
Stanford University
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| Department |
Institute for Stem Cell Biology and Regenerative Medicine
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| Lab |
Majeti
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| Street address |
Room 3005, 265 Campus Drive, 3rd Floor
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL19883 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Broad GenePattern] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA277970 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66792_RAW.tar |
71.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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