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Series GSE66913 Query DataSets for GSE66913
Status Public on Jan 13, 2016
Title Grape_Bud_Dormancy
Platform organism Vitis vinifera
Sample organisms Vitis riparia; Vitis hybrid cultivar
Experiment type Expression profiling by array
Summary Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american species) was compared under long and short photoperiod. Three separate replicates (5 plants/replicate) were treated in each of 2 separate years (2007 and 2008) to generate paradormant (LD) and same aged endodormancy-induced (SD) buds for transcriptomic, proteomic and metabolomic analysis. Potted, spur-pruned two to six-year-old vines were removed from cold storage (Seyval 3-19-07, 3/18/08; V. riparia 3/26/07, 3/24/08) and grown under a LD (15 h) at 25/20 + 3C day/night temperatures (D/N). When vines reached 12-15 nodes they were randomized into groups for differential photoperiod treatments. On 4/30/07 and 4/28/08 LD and SD (13 h) treatments were imposed with automated photoperiod system (VRE Greenhouse Systems). Temperatures were maintained at 25/20 + 3C D/N. Three replications (5 vines/replication) were harvested between 5/07-6/07 and then again in 5/08-6/08. At 1, 3, 7, 14, 21, 28 and 42 days of differential photoperiod treatment, buds were harvested from nodes 3 to 12 (from the base of the shoot) of each separate replicate, immediately frozen in liquid nitrogen, and placed at -80C for future RNA, protein and metabolite extraction. These time points encompass early reversible phases as well as key time points during transition to irreversible endodormancy development. After photoperiod treatments and bud harvests, all pruned vines were returned to LD and monitored for bud endodormancy. The endodormant vines were identified after 28 days and moved to cold storage. The nondormant vines were allowed to grow again and induced into dormancy at a later date. Acknowledgement:This study was funded by NSF Grant DBI0604755 and funds from the South Dakota Agriculture Experiment Station. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Anne Fennell. The equivalent experiment is VV18 at PLEXdb.]
 
Overall design genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 1 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 3 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 7 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 14 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 21 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 28 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 42 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 1 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 3 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 7 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 14 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 21 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 28 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 13 h - time: 42 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 1 days(2-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 3 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 7 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 14 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 21 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 28 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 15 h - time: 42 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 1 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 3 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 7 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 14 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 21 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 28 days(3-replications); genotype: Seyval (SV, Seyve Villard 5-276) - year: 2 - photoperiod: 13 h - time: 42 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 1 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 3 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 7 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 14 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 21 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 28 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 15 h - time: 42 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 1 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 3 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 7 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 14 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 21 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 28 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 1 - photoperiod: 13 h - time: 42 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 1 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 3 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 7 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 14 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 21 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 28 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 15 h - time: 42 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 1 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 3 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 7 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 14 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 21 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 28 days(3-replications); genotype: V. riparia (VR, PI588259) - year: 2 - photoperiod: 13 h - time: 42 days(3-replications)
Web link http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=VV18
 
Contributor(s) Fennell A, Fennell A
Citation(s) 26582400
Submission date Mar 16, 2015
Last update date Jun 22, 2017
Contact name PLEXdb Curator, Sudhansu Dash
Organization name Iowa State University
Department Virtual Reality Application Center
Lab PLEXdb
Street address 1023 Crop Genomes Informatics Lab (Iowa State University)
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL1320 [Vitis_Vinifera] Affymetrix Vitis vinifera (Grape) Genome Array
Samples (167)
GSM1634094 genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 1 days - rep1
GSM1634095 genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 1 days - rep2
GSM1634096 genotype: Seyval (SV, Seyve Villard 5-276) - year: 1 - photoperiod: 15 h - time: 1 days - rep3
Relations
BioProject PRJNA278360

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66913_RAW.tar 329.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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