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| Status |
Public on May 13, 2016 |
| Title |
Genome-wide analysis of DNA methylation in the sexual stage of the insect pathogenic fungus Cordyceps militaris |
| Organism |
Cordyceps militaris |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Genome-wide analysis of DNA methylation for asexual and sexual stages of Cordyceps militaris
DNA methylation is a basic epigenetic mechanism found in eukaryotes, but its patterns and roles vary significantly among diverse taxa. In fungi, DNA methylation has various effects on diverse biological processes; however, its function underlying the sexual development of fungi remains unclear. Cordyceps militaris, readily performing sexual reproduction, provides a remarkably rich model for understanding epigenetic processes in sexual development. The methylome was surveyed to assess DNA methylation patterns of the nascent fruiting body in C. militaris at single-base resolution by genomic bisulfite sequencing (BS-Seq). The results showed that around 0.4 % of cytosines are methylated, and that there is no difference compared with the DNA methylation level (0.39 %) during asexual development. However, 225 differentially methylated regions (DMRs) were identified between the stages. Moreover, RNA-Seq analysis indicated that the DMRs have no direct relation with the genes for fungal sexual development in C. militaris. DNA methylation regions from the BS-Seq show a striking correspondence to regions predicted to be repetitive and repeat-induced point mutation (RIP) mutated, suggesting that DNA methylation functions as a genome defense mechanism in sexual development. These results provide a comprehensive characterization of DNA methylation in the sexual development of C. militaris, which will contribute to future investigations of epigenetics in fungi.
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| Overall design |
Bisulphite converted genomic DNA from the mycelium and fruitingbody of Cordyceps militaris
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| Contributor(s) |
Wang Y, Huang B |
| Citation missing |
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| |
| Submission date |
Mar 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yulong Wang |
| E-mail(s) |
yulong4444@126.com
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| Organization name |
Anhui Agricultural University
|
| Lab |
Anhui Provincial Key Laboratory of Microbial Pest Control
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| Street address |
130 West Changjiang Road
|
| City |
Hefei |
| ZIP/Postal code |
230036 |
| Country |
China |
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| Platforms (1) |
| GPL19894 |
Illumina HiSeq 2000 (Cordyceps militaris) |
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| Samples (2) |
| GSM1634321 |
genomic DNA from mycelium of cordyceps militaris |
| GSM1634322 |
genomic DNA from fruitingbody of cordyceps militaris |
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| Relations |
| BioProject |
PRJNA278366 |
| SRA |
SRP056198 |
