|
| Status |
Public on Nov 02, 2015 |
| Title |
Role of miRNA in inflammatory bowel disease |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Cytomegalovirus; Lymphocryptovirus; Rhadinovirus; JC polyomavirus; Human immunodeficiency virus 1; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
This is a miRNA expression analysis by microarray. Comparison was made beween Ulcerative Colitis (UC) vs control, Active UC vs Remission, Ascending Colon vs Rectosigmoid area of Colon. The samples were labeled using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ and hybridized on the miRCURY LNA™ microRNA Array (7th gen) following the scheme you outlined in the sample submission form. The technical data quality assessment showed that the labeling was successful as all capture probes for the control spike-in oligo nucleotides produced signals in the expected range. Following normalization of the quantified signals (background corrected) using the global Lowess regression algorithm, we have performed an unsupervised as well as supervised data analysis.
|
| |
|
| Overall design |
RNA from 4 samples pooled in each category. NA- Sample from ascending colon Active Ulcerative colitis; RA- Sample from rectosigmoid colon Active Ulcerative colitis; NC- Sample from ascending colon Control individuals; RC-Sample from rectosigmoid colon Control individuals; NR- Sample from ascending colon remission Ulcerative colitis; RR- Sample from rectosigmoid colon remission Ulcerative colitis; Comparision was done in UC vs Control, Ascending Colon vs Rectosigmoid area in UC and Ascending Colon vs Rectosigmoid area in Controls
The supplementary file samplesinfo_2146-1.csv includes additional information about the raw data files
|
| |
|
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 16, 2015 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Raju Ranjha |
| E-mail(s) |
raju_ranjha@yahoo.co.in
|
| Organization name |
Jawaharlala Nehru University
|
| Department |
School of Life Sciences
|
| Lab |
441
|
| Street address |
Lab. No.441, School of Life Sciences,
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
New Delhi-110067 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL19128 |
Exiqon miRCURY LNA microRNA array; 7th generation - hsa, mmu & rno (miRBase 18.0) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA278396 |
Less...
More...