|
| Status |
Public on Sep 14, 2015 |
| Title |
Gata6 induced XEN-like cells w/o Sox7 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand the role of Sox7 in primitive endoderm differentiation, we compare the gene expression pattern of Sox7 (+/-) and Sox7 (-/-) ES cells with or without dexamethasome (Dex) treatment. Because these ES cells harbour Gata6-GR transgene, Dex treatment forces ES cells differentate into XEN-like cells. As Sox7 (-/-) ES cells can differentiate into XEN-like cell by morphology, we assessed genome wide gene expression pattern.
|
| |
|
| Overall design |
Sox7 (+/-) ES cells and Sox7 (-/-) ES cells are forced to differentiate into XEN-like cells by Gata6-GR transgene. To compare the gene expression, we collected RNA samples at day4 with or without dexamethasone treatment from each genotype.
|
| |
|
| Contributor(s) |
Kinoshita M, Yamane M, Niwa H |
| Citation(s) |
26475439 |
| Submission date |
Mar 17, 2015 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Hitoshi Niwa |
| E-mail(s) |
niwa@kumamoto-u.ac.jp
|
| Organization name |
RIKEN
|
| Department |
Center for Developmental Biology
|
| Lab |
Laboratory for Pluripotent Stem Cell Studies
|
| Street address |
2-2-3 Minatojima-minamimachi,Chuo-ku
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA278525 |
Less...
More...