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| Status |
Public on Mar 20, 2015 |
| Title |
Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica |
| Organism |
Musca domestica |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sex determination evolves rapidly, often because of turnover of the genes at the top of the pathway. The house fly, Musca domestica, has a multifactorial sex determination system, allowing us to identify the selective forces responsible for the evolutionary turnover of sex determination in action. There is a male determining factor, M, on the Y chromosome (Y^M), which is probably the ancestral state. An M factor on the third chromosome (III^M) has reached high frequencies in multiple populations across the world, but the evolutionary forces responsible for the invasion of III^M are not resolved. To test if the III^M chromosome invaded because of sex-specific selection pressures, we used mRNA sequencing to determine if isogenic males that differ only in the presence of the Y^M or III^M chromosome have different gene expression profiles. We find that more genes are differentially expressed between Y^M and III^M males in testis than head, and that genes with male-biased expression are most likely to be differentially expressed between Y^M and III^M males. This suggests that male phenotypes, especially those related to male fertility, are more likely to be affected by the male-determining chromosome, supporting the hypothesis that sex-specific selection acts on alleles linked to the male-determining locus driving evolutionary turnover in the sex determination pathway. We additionally find that III^M males have a "masculinization" gene expression profile, suggesting that the III^M chromosome has accumulated an excess of male-beneficial alleles because of its male-limited transmission.
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| Overall design |
3 replicates of testes and male heads from a Y^M and a III^M strain, and one sample of head and ovary from each of the strains
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| Contributor(s) |
Meisel RP, Scott JG, Clark AG |
| Citation(s) |
26142430 |
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| Submission date |
Mar 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Meisel |
| Organization name |
University of Houston
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| Department |
Biology and Biochemistry
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| Street address |
3455 Cullen Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77204-5001 |
| Country |
USA |
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| Platforms (1) |
| GPL19912 |
Illumina HiSeq 2500 (Musca domestica) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA278841 |
| SRA |
SRP056323 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67065_RNAseq_results.tsv.gz |
1.4 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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