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| Status |
Public on Mar 21, 2015 |
| Title |
Expression data from KSL and GMP cells of FLT3/ITD and FLT3/ITD-SmoM2 mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
FLT3/ITD-SmoM2 mice developed rapidly fatal myeloid leukemia compared to FLT3/ITD only mice, suggesting that overactivation of the Hedgehog signaling pathway via SmoM2 can drive myeloid disease progression
We used the Affymetrix Mouse 430_2.0 microarray to detail global gene expression responsible for disease progression in sorted bone marrow cells and found that the Hedgehog signaling pathway contributes to disease progression by enhancing FLT3 signaling
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| Overall design |
KSL and GMP cells were FACS sorted from wild-type, FLT3/ITD and FLT3/ITD-SmoM2 bone marrow when the mice were around 3 months of age, when the FLT3/ITD-SmoM2 mice developed leukemia. RNA was extracted from the sorted cells and hybridized onto the Mouse 430_2.0 Affymetrix array. Wild-type and FLT3/ITD mice that were used as controls were littermates of the FLT3/ITD-SmoM2 leukemic mice. Each sample consists of pooled cells from 3 mice.
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| Contributor(s) |
Lim Y, Matsui W |
| Citation(s) |
26062848 |
| |
| Submission date |
Mar 20, 2015 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Yiting Lim |
| Organization name |
Johns Hopkins University School of Medicine
|
| Street address |
1650 Orleans Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM1639867 |
GMP cells from wild type mouse bone marrow |
| GSM1639868 |
KSL cells from wild type bone marrow |
| GSM1639869 |
GMP cells from FLT3/ITD mouse bone marrow |
| GSM1639870 |
KSL cells from FLT3/ITD bone marrow |
| GSM1639871 |
GMP cells from FLT3/ITD-SmoM2 mouse bone marrow |
| GSM1639872 |
KSL cells from FLT3/ITD-SmoM2 mouse bone marrow |
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| Relations |
| BioProject |
PRJNA279028 |
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