|
Status |
Public on Feb 15, 2007 |
Title |
DNA methylation, RNA Pol2 binding and H3-K4me2 of human promoters in WI38 primary fibroblasts and sperm cells |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by genome tiling array Methylation profiling by genome tiling array
|
Summary |
To gain insights into the function of DNA methylation at cis-regulatory regions and its impact on gene expression, we measured methylation, RNA polymerase II and histone modifications at 24.000 promoters in primary human soma and germline cells. Keywords: DNA methylation, MeDIP
|
|
|
Overall design |
The chosen array from NIMBLEGEN (HG17_min_promoter array) represents 24134 putative human promoters, each covered by 15 oligonucleotides spanning 1.3 kb upstream and 0.2 kb downstream of the transcription start site. All experiments have been repeated two or three times, including one dye-swop.
|
|
|
Contributor(s) |
Weber M, Stadler MB, Rebhan M, Schubeler D |
Citation(s) |
17334365 |
|
Submission date |
Jan 11, 2007 |
Last update date |
Mar 16, 2012 |
Contact name |
Michaël Weber |
E-mail(s) |
michael.weber@fmi.ch
|
Organization name |
Friedrich Miescher Institute
|
Department |
Epigenetics
|
Lab |
Dirk Schübeler
|
Street address |
Maulbeerstrasse 66
|
City |
Basel |
ZIP/Postal code |
CH 4058 |
Country |
Switzerland |
|
|
Platforms (1) |
GPL3497 |
NimbleGen hg17 Human 1.5 Kb MinPromoter array |
|
Samples (11)
|
GSM154834 |
human sperm cells_MeDIP_Replicate2 |
GSM154835 |
human sperm cells_MeDIP_Replicate3 |
GSM154836 |
WI38 cells_ChIP RNA Pol2_Replicate1 |
GSM154837 |
WI38 cells_ChIP RNA Pol2_Replicate2 |
GSM154838 |
WI38 cells_ChIP RNA Pol2_Replicate3 |
GSM154840 |
WI38 cells_ChIP H3-K4me2_Replicate1 |
GSM154842 |
WI38 cells_ChIP H3-K4me2_Replicate2 |
GSM154844 |
WI38 cells_ChIP H3-K4me2_Replicate3 |
|
Relations |
BioProject |
PRJNA99041 |