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| Status |
Public on Sep 01, 2015 |
| Title |
Analysis of the role of miR-21 in liver regeneration after partial hepatectomy (PHx) in chronic ethanol-treated rats through in vivo inhibition using LNAs |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we analyzed the role of miR-21 in liver regeneration after partial hepatectomy (PHx) in chronic ethanol-treated rats. Male Sprague-Dawley rats were fed a liquid diet containing 36% of total calories derived from ethanol for 5 weeks; corresponding pair-fed calorie-matched controls were fed diets in which ethanol calories were replaced by carbohydrate. After 5 weeks, locked nucleic acid (LNA)-modified oligo antisense to miR-21 (AM21, Exiqon, Vedbaek, Denmark) was used to inhibit miRNA in vivo, and rats were subjected to 70% PHx. Liver samples were collected at 24h after the surgery. The excised liver samples at t=0 served as within-animal controls. Rat Gene 2.0 ST (Affymetrix, Santa Clara, CA) arrays were used to obtain global gene expression data from pooled liver samples (pools of 3 or 4 biological replicates/array, total 8 arrays).
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| Overall design |
Gene expression was profiled in AM21 treated or untreated chronic ethanol-fed (EtOH) and carbohydrate control pair-fed (CHO) rat liver prior to (control, t=0) and 24 h after 70% partial hepatectomy (PHx).
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| Contributor(s) |
Dippold R, Vadigepalli R, Hoek JB |
| Citation(s) |
27634014 |
| Submission date |
Mar 24, 2015 |
| Last update date |
Feb 07, 2019 |
| Contact name |
Egle Juskeviciute |
| Organization name |
Thomas Jefferson University
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| Department |
Pathology
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| Street address |
1020 Locust st. JAH 527
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL17117 |
[RaGene-2_0-st] Affymetrix Rat Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA279310 |
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