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Series GSE67365 Query DataSets for GSE67365
Status Public on Mar 28, 2015
Title Expression data from mouse intestinal epithelial cells infected with Trichinella spiralis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Trichinella spiralis is a highly destructive parasitic nematode that invades and destroys intestinal epithelial cells, injures many different tissues during its migratory phase, and occupies and transforms myotubes during the final phase of its life cycle. Mice deficient in the IL-1 family receptor for the DAMP, IL-33 (called ST2), display reduced intestinal Th2 responses and impaired mast cell activation. IL-33 was constitutively expressed in intestinal epithelial cells, where it became concentrated in nuclei within 2 days of infection. Nuclear localization was an innate response to infection that occurred in intestinal regions where worms were actively migrating. We isolated intestinal epithelial cells from uninfected mice (cytoplasmic IL-33) and mice at 2 days post-infection (nuclear IL-33) to compare global expression profiles.
We used microarrays to characterize the global gene expression that occurs in intestinal epithelial cells following T. spiralis-induced nuclear translocation of IL-33.
 
Overall design Intestinal epithelial cells were isolated from Rag2-/- mice at day zero (uninfected) or two days post-infection with T. ispiralis for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Blum LK, Appleton JA
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Submission date Mar 27, 2015
Last update date Oct 24, 2023
Contact name Lisa K Blum
E-mail(s) lkblum@gmail.com
Phone 805-234-7162
Organization name Stanford University
Lab William H. Robinson
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM1645538 mm4239.U
GSM1645539 mm4241.U
GSM1645540 mm4269.U
Relations
BioProject PRJNA279591

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Supplementary file Size Download File type/resource
GSE67365_RAW.tar 425.2 Mb (http)(custom) TAR (of CEL, DAT, XML)
Processed data not provided for this record

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