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| Status |
Public on May 28, 2015 |
| Title |
RNA sequencing of cells treated with DMSO or Retinoic acid during cardiac differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analysis of transcriptional differences between control and RA-treated cells during cardiac differentiation. The hypothesis tested in these samples is that addition of RA during differentiation towards atrial-like cardiomyocytes while control cells treated with DMSO result in ventricular-like cardiomyocytes.
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| Overall design |
NKX2.5 (eGFP/w)-hESCs were differentiated to cardiomyocytes with spin EB protocol, with the addition of RA or DMSO. Cells were sorted at day-31 based on GFP resulting in CTplus, CTminus, RAplus or RAminus goups. RNA was isolated from each of these fractions for sequencing.
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| Contributor(s) |
Roost MS, van Iperen L, Ariyurek Y, Buermans HP, Arindrarto W, Devalla HD, Passier R, Mummery CL, Carlotti F, de Koning EJ, van Zwet EW, Goeman JJ, de Sousa Lopes SM |
| Citation(s) |
26028532 |
| Submission date |
Apr 14, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Nannan He |
| E-mail(s) |
N.He@lumc.nl
|
| Organization name |
Leiden University Medical Center
|
| Street address |
Einthovenweg 20
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA281093 |
| SRA |
SRP057156 |
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