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Status |
Public on Feb 27, 2007 |
Title |
Gene expression analysis of bronchial epithelial cells stimulated by different airway pathogens. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Bronchial epithelial cells represent the first line of defense against invading airborne pathogens. They are important contributors to innate mucosal immunity and provide a variety of anti-microbial effectors. To investigate the role of epithelial cells upon infection of airway pathogens, we stimulated BEAS-2B cells for 4 h with UV-inactivated bronchial pathogens including Staphylococcus aureus, Pseudomonas aeruginosa and Respiratory Syncitial Virus (RSV) that among other receptors can strongly activate TLR2, TLR4 and TLR3, respectively. Keywords: expression profiling, response to pathogens
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Overall design |
All conditions were done in triplicates except for Staphylococcus aureus, were two replicates were done. As a control, unstimulated BEAS-2B were used. Altogether 11 arrays were hybridized.
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Contributor(s) |
Mayer AK, Muehmer M, Mages J, Gueinzius K, Hess C, Heeg K, Bals R, Lang R, Dalpke AH |
Citation(s) |
17312161 |
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Submission date |
Jan 19, 2007 |
Last update date |
Dec 06, 2018 |
Contact name |
Joerg Mages |
E-mail(s) |
mages@lrz.tum.de
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Organization name |
Technical University Munich
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Department |
Institute of Medical Microbiology, Immunology and Hygiene
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Street address |
Trogerstrasse 30
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City |
Munich |
ZIP/Postal code |
81675 |
Country |
Germany |
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Platforms (1) |
GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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Samples (11)
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Relations |
BioProject |
PRJNA99201 |