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Series GSE68172 Query DataSets for GSE68172
Status Public on Apr 23, 2015
Title LAA expression profiles on LSC compared to other tissues
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Leukemic stem cells (LSC) might be the source for leukemic disease self-renewal and account for disease relapse after treatment, which makes them a critical target for further therapeutic options. Leukemia associated antigens (LAA) might be suitable structures to be attacked by immunotherapeutic agents. We performed primary AML sample enrichment and microarray studies to define LAA expression levels in AML. We compared the LAA expression in the enriched CD34+CD38- AML fraction to that of enriched HSC of healthy donors and AML bulk cells (CD34+CD38+, CD34-CD38+ and CD34-CD38). Furthermore, we investigated the expression patterns of co-stimulatory molecules in LSC, bulk AML cells and enriched HSC, Conclusion: We demonstrated the differential expression of several LAA in LSC, and their suitability as target structures.
We enriched primary AML samples using CD34 and CD38 as markers to compare LAA expression levels of LSC, HSC and AML bulk.
 
Overall design LAA expression profiles comparing LSC, HSC and leukemic bulk AML

citation: Leukemic progenitor cells are susceptible to targeting by stimulated cytotoxic T cells against immunogenic leukemia-associated antigens
Vanessa Schneider, Lu Zhang, Markus Rojewski, Natalie Fekete, Hubert Schrezenmeier, Alexander Erle, Lars Bullinger, Susanne Hofmann, Marlies Götz, Konstanze Döhner, Susann Ihme, Hartmut Döhner, Christian Buske, Michaela Feuring-Buske, Jochen Greiner
 
Contributor(s) Schneider V, Greiner J
Citation(s) 25912930
Submission date Apr 22, 2015
Last update date Jan 02, 2024
Contact name Jochen Greiner
E-mail(s) greiner@diak-stuttgart.de
Organization name University Clinic Ulm
Department Internal Medicine III
Lab Tumorimmunologie Ulm
Street address Helmholzstrasse 10
City Ulm
ZIP/Postal code 89075
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (77)
GSM1664972 sample 1
GSM1664973 sample 2
GSM1664974 sample 3
Relations
BioProject PRJNA281951

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68172_RAW.tar 367.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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