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Series GSE68200 Query DataSets for GSE68200
Status Public on Apr 20, 2016
Title Ribosome Profiling Reveals Translational Control During the Caulobacter crescentus cell cycle
Organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by high throughput sequencing
Summary The Caulobacter cell cycle includes sequential differentiation of the cell poles and an asymmetric cell division yielding distinct daughter cells. RNA-seq and ribosome profiling were used to measure global messenger RNA (mRNA) abundance and translation levels throughout the Caulobacter cell cycle revealing translational control in 51% of cell cycle-regulated genes. Ribosome profiling levels were temporally regulated for ten proteins encoding asymmetrically localized multi-protein assemblies that specify cell fate upon cell division, with five ORFs being regulated with a >2 fold contribution from the translation efficiency. Individual ORFs within 198 operons undergo different cell cycle patterns of translation efficiency, allowing decoupling of the expression of these co-transcribed units. This work reveals that, in addition to transcriptional control, there is prevalent translational control of cell cycle-regulated genes, providing a framework for a multi-level program of cell cycle control that generates daughter cells of different fates.
Overall design Ribosome profiling and RNA-seq data were collected in sychronized Caulobacter crescentus NA1000 cells grown in M2G to measure changes in mRNA levels and translation levels during the asymmetric cell division cycle.
Contributor(s) Schrader JM, Li G, Jonathan WS, Lucy S
Citation(s) 27791168
Submission date Apr 23, 2015
Last update date May 15, 2019
Contact name Jared Michael Schrader
Organization name Wayne State University
Department Biological Sciences
Lab Schrader Lab
Street address 5047 Gullen Mall, Biological Sciences Building Room 2168
City Detroit
State/province MI
ZIP/Postal code 48202
Country USA
Platforms (1)
GPL18276 Illumina HiSeq 2000 (Caulobacter crescentus NA1000)
Samples (12)
GSM1665588 swarmer M2G RNA-seq
GSM1665589 early stalk M2G RNA-seq
GSM1665590 late stalk M2G RNA-seq
BioProject PRJNA282016
SRA SRP057599

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Supplementary file Size Download File type/resource
GSE68200_RAW.tar 66.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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