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| Status |
Public on Apr 20, 2016 |
| Title |
Ribosome Profiling Reveals Translational Control During the Caulobacter crescentus cell cycle |
| Organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The Caulobacter cell cycle includes sequential differentiation of the cell poles and an asymmetric cell division yielding distinct daughter cells. RNA-seq and ribosome profiling were used to measure global messenger RNA (mRNA) abundance and translation levels throughout the Caulobacter cell cycle revealing translational control in 51% of cell cycle-regulated genes. Ribosome profiling levels were temporally regulated for ten proteins encoding asymmetrically localized multi-protein assemblies that specify cell fate upon cell division, with five ORFs being regulated with a >2 fold contribution from the translation efficiency. Individual ORFs within 198 operons undergo different cell cycle patterns of translation efficiency, allowing decoupling of the expression of these co-transcribed units. This work reveals that, in addition to transcriptional control, there is prevalent translational control of cell cycle-regulated genes, providing a framework for a multi-level program of cell cycle control that generates daughter cells of different fates.
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|
| Overall design |
Ribosome profiling and RNA-seq data were collected in sychronized Caulobacter crescentus NA1000 cells grown in M2G to measure changes in mRNA levels and translation levels during the asymmetric cell division cycle.
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| Contributor(s) |
Schrader JM, Li G, Jonathan WS, Lucy S |
| Citation(s) |
27791168 |
| |
| Submission date |
Apr 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jared Michael Schrader |
| E-mail(s) |
schrader@wayne.edu
|
| Organization name |
Wayne State University
|
| Department |
Biological Sciences
|
| Lab |
Schrader Lab
|
| Street address |
5047 Gullen Mall, Biological Sciences Building Room 2168
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48202 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18276 |
Illumina HiSeq 2000 (Caulobacter crescentus NA1000) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA282016 |
| SRA |
SRP057599 |
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