|Public on Apr 20, 2016
|Ribosome Profiling Reveals Translational Control During the Caulobacter crescentus cell cycle
|Caulobacter vibrioides NA1000
|Expression profiling by high throughput sequencing
|The Caulobacter cell cycle includes sequential differentiation of the cell poles and an asymmetric cell division yielding distinct daughter cells. RNA-seq and ribosome profiling were used to measure global messenger RNA (mRNA) abundance and translation levels throughout the Caulobacter cell cycle revealing translational control in 51% of cell cycle-regulated genes. Ribosome profiling levels were temporally regulated for ten proteins encoding asymmetrically localized multi-protein assemblies that specify cell fate upon cell division, with five ORFs being regulated with a >2 fold contribution from the translation efficiency. Individual ORFs within 198 operons undergo different cell cycle patterns of translation efficiency, allowing decoupling of the expression of these co-transcribed units. This work reveals that, in addition to transcriptional control, there is prevalent translational control of cell cycle-regulated genes, providing a framework for a multi-level program of cell cycle control that generates daughter cells of different fates.
|Ribosome profiling and RNA-seq data were collected in sychronized Caulobacter crescentus NA1000 cells grown in M2G to measure changes in mRNA levels and translation levels during the asymmetric cell division cycle.
|Schrader JM, Li G, Jonathan WS, Lucy S
|Apr 23, 2015
|Last update date
|May 15, 2019
|Jared Michael Schrader
|Wayne State University
|5047 Gullen Mall, Biological Sciences Building Room 2168
|Illumina HiSeq 2000 (Caulobacter crescentus NA1000)