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| Status |
Public on Aug 31, 2015 |
| Title |
miR-1246 association with distant metastasis and poor prognosis in breast cancer patients |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Circulating microRNAs have recently emerged as a new class of promising non-invasive cancer biomarkers. The purpose of this study was the identification by a high-throughput approach (miRNA microarray) of circulating miRNAs associated with breast cancer-derived distant metastasis. To achieve this goal, we resorted to archival plasma samples collected from patients in the control arm of a randomized clinical trial on stage I breast cancer. We compared plasma miRNA levels in patients that developed distant metastasis after a radical or conservative surgery and in those long-term disease-free. Microarray results were technically and independently validated by Real Time PCR. Subsequent in vitro, in vivo and in silico analyses were performed to investigate the miRNA biological/clinical significance in relation with breast cancer progression. We demonstrated that high circulating miR-1246 levels were associated to distant metastasis and that high tissue expression of this miRNA was correlated with unfavorable outcome in ER+HER2- breast cancer patients. In addition, miR-1246 expression was also found to be related with stem-like features.
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| Overall design |
64 samples (32 patients with metastasis and 32 patients with no metastasis) were considered from a total of 208 hybridized samples collected between 1987 and 2004 (including 94 paired samples according to tumor ER status, age at drawing, drawing year, time from surgery, 7 additional samples from 'no evidence of disease' (NED) patients, 10 references, 1 healthy donor and 2 replicated samples)
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| Contributor(s) |
Landoni E, Callari M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 28, 2015 |
| Last update date |
Sep 01, 2015 |
| Contact name |
Elena Landoni |
| E-mail(s) |
elena.landoni@istitutotumori.mi.it
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| Organization name |
Fondazione IRCCS Istituto Nazionale dei Tumori
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| Street address |
Via Vanzetti 5
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| City |
Milano |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL16770 |
Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray (miRBase release 16.0 miRNA ID version) |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA282533 |
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