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Series GSE68430 Query DataSets for GSE68430
Status Public on May 01, 2015
Title Molecular alterations in areas generating fast ripples in an animal model of temporal lobe epilepsy
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The molecular basis of epileptogenesis is poorly characterized. Studies in humans and animal models have identified an electrophysiological signature that precedes the onset of epilepsy, which has been termed fast ripples (FRs) based on its frequency. Multiple lines of evidence implicate regions generating FRs in epileptogenesis, and FRs appear to demarcate the seizure onset zone, suggesting a role in ictogenesis as well. We performed gene expression analysis comparing areas of the dentate gyrus that generate FRs to those that do not generate FRs in a well-characterized rat model of epilepsy. We identified a small cohort of genes that are differentially expressed in FR versus non-FR brain tissue and used quantitative PCR to validate some of those that modulate neuronal excitability. Gene expression network analysis demonstrated conservation of gene co-expression between non-FR and FR samples, but examination of gene connectivity revealed changes that were most pronounced in the cm-40 module, which contains several genes associated with synaptic function and the differentially expressed genes Kcna4, Kcnv1, and Npy1r that are down-regulated in FRs. We then demonstrate that the genes within the cm-40 module are regulated by seizure activity and enriched for the targets of the RNA binding protein Elavl4. Our data suggest that seizure activity induces co-expression of genes associated with synaptic transmission and that this pattern is attenuated in areas displaying FRs, implicating the failure of this mechanism in the generation of FRs.
 
Overall design 21 samples, 10 fast ripple areas and 11 paired non-fast ripples areas
 
Contributor(s) Winden K
Citation(s) 25818007
Submission date Apr 30, 2015
Last update date May 02, 2015
Contact name Kellen Winden
Organization name Boston Children's Hospital
Department Neurology
Street address 300 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL6101 Illumina ratRef-12 v1.0 expression beadchip
Samples (21)
GSM1671593 Dentate gyrus - Fast ripple [P91_S1_E]
GSM1671594 Dentate gyrus - Non-fast ripple [P91_S1_NE]
GSM1671595 Dentate gyrus - Fast ripple [P91_S3_E]
Relations
BioProject PRJNA282722

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68430_RAW.tar 2.8 Mb (http)(custom) TAR
GSE68430_non_normalized.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table
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