|
| Status |
Public on Apr 12, 2017 |
| Title |
DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells |
| Organisms |
Mus musculus; Mus musculus musculus x Mus musculus castaneus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Blastocyst-derived embryonic stem cells (ESCs) and gonad-derived embryonic germ cells (EGCs) represent two classic types of pluripotent cell lines, yet their molecular equivalence remains incompletely understood. Here, we compare genome-wide methylation patterns between isogenic ESC and EGC lines to define epigenetic similarities and differences. Surprisingly, we find that sex rather than cell type drives methylation patterns in ESCs and EGCs. Cell fusion experiments further reveal that the ratio of X chromosomes to autosomes dictates methylation levels, with female hybrids being hypomethylated and male hybrids being hypermethylated. We show that the X-linked MAPK phosphatase DUSP9 is upregulated in female compared to male ESCs, and its heterozygous loss in female ESCs leads to male-like methylation levels. However, male and female blastocysts are similarly hypomethylated, indicating that sex-specific methylation differences arise in culture. Collectively, our data demonstrate the epigenetic similarity of sex-matched ESCs and EGCs and identify DUSP9 as a regulator of female-specific hypomethylation.
|
| |
|
| Overall design |
Genetically matched mouse EGC and ESC lines were collected for DNA methylation analysis via mRRBS
|
| |
|
| Contributor(s) |
Choi J, Webster J, Boyle P, Clement K, Meissner A, Hochedlinger K, Gu H |
| Citation(s) |
28366588, 28746311 |
| Submission date |
May 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kendell Clement |
| Organization name |
Harvard University
|
| Lab |
Meissner Lab
|
| Street address |
7 Divinity Ave.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL22270 |
Illumina HiSeq 2500 (Mus musculus musculus x M. m. castaneus) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA283520 |
| SRA |
SRP058183 |
Less...
More...