 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 25, 2015 |
| Title |
Leukemia Inhibitory Factor in C26 Cancer Cachexia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cachexia is an exacerbating event in many types of cancer that is strongly associated with a poor prognosis. We have identified cytokine, signaling and transcription factors that are required for cachexia in the mouse C26 colon carcinoma model of cancer. C2C12 myotubes treated with conditioned medium from C26 cancer cells induced atrophy and activated a STAT-dependent reporter gene but not reporter genes dependent on SMAD, FOXO, C/EBP, NF-ĸB, or AP-1. Of the gp130 family members IL-11, IL-6, oncostatin M (OSM), and leukemia inhibitory factor (LIF), only OSM and LIF were sufficient to activate the STAT reporter in myotubes. A LIF blocking antibody abolished C26 CM-induced STAT reporter activation STAT3 phosphorylation and myotube atrophy, but blocking antibodies to IL-6 or OSM did not. JAK2 inhibitors also blocked the C26 CM-induced STAT reporter activation, STAT3 phosphorylation, and atrophy in myotubes. LIF at levels found in the C26 CM was sufficient for STAT reporter activation and atrophy in myotubes. In vivo, an increase in serum LIF preceded the increase in IL-6 in mice with C26 tumors. Overexpression of a dominant negative Stat3Cβ-EGFP gene in myotubes and in mouse muscle blocked the atrophy caused by C26 CM or C26 tumors, respectively. Taken together these data support an important role of LIF- JAK2-STAT3 in C26 cachexia and point to a therapeutic approach for at least some types of cancer cachexia.
|
| |
|
| Overall design |
from three replicate wells of cells at each treatment, pools of total RNA were used to create cDNA which were evaluated on Affymetrix mouse gene 1.0 ST v.1 arrays.
|
| |
|
| Contributor(s) |
Seto DN, Kandarian SC, Jackman RW |
| Citation(s) |
26092726 |
| |
| Submission date |
May 13, 2015 |
| Last update date |
Jun 25, 2015 |
| Contact name |
Robert William Jackman |
| E-mail(s) |
rjackman@bu.edu
|
| Phone |
6173532719
|
| Organization name |
Boston University (BU)
|
| Department |
Health Sciences
|
| Street address |
635 Commonwealth Avenue/rm 444
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19485 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_16.0.0] |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA283877 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68827_C26CM_4h_8h_24h_foldchange.txt.gz |
799.0 Kb |
(ftp)(http) |
TXT |
| GSE68827_LIF_2015-03-12_4h_8h_foldchange.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| GSE68827_LIF_2015-04-30_24h_foldchange.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE68827_RAW.tar |
69.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...