|
| Status |
Public on May 15, 2015 |
| Title |
Gene expression profiles of control and EWS-FLI1-transduced neural crest stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression profiles were generated from hESC-derived neural crest stem cells following transduction with GFP control vector or EWS-FLI1 vector. Expression was analyzed in stem cell conditions 5 days after transduction (undifferentiated conditions) and after 6 weeks in differentiation media (differentiation conditions).
Changes in gene expression over time were compared between control and EWS-FLI1 expresssing cells.
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| |
|
| Overall design |
Total RNA was extracted from 3 replicates for each of 4 conditions (undifferentiated control, undifferentiated EWS-FLI1, differentiated control and differentiated EWS-FLI1. Samples were analyzed by Affymetrix exon arrays using standard procedures.
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| |
|
| Contributor(s) |
Lawlor ER |
| Citation(s) |
25625846, 27363011 |
| |
| Submission date |
May 14, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Elizabeth R Lawlor |
| E-mail(s) |
elawlor@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1600 Huron Parkway, NCRC Bldg 520
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2800 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (12)
|
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| Relations |
| BioProject |
PRJNA283989 |
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