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| Status |
Public on May 15, 2015 |
| Title |
Transcriptomic analysis of Clostridium acetobutylicum biofilm cells adsorbed onto fibrous matrix in biobutanol production [II] |
| Platform organisms |
Arthrobacter sp.; Clostridium acetobutylicum DSM 1731 |
| Sample organism |
Clostridium acetobutylicum |
| Experiment type |
Expression profiling by array
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| Summary |
Immobilization of Clostridium acetobutylicum B3 onto fibrous matrix by surface-adsorption was developed and applied to biobutanol production. The immobilized C. acetobutylicum B3 cells formed biofilm and showed dramatically improved butanol tolerance and production rate. DNA array-based transcriptional analysis of C.acetobutylicum B3 biofilm cells was conducted to elucidate the gene expression profile of the biofilm cells. Results showed that about 16% of the whole genome was differentially expressed. The most apparently differentially expressed genes were involved in amino acid transport and metabolism, inorganic ion transport and metabolism, energy production and conversion, and coenzyme transport and metabolism.
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| Overall design |
Samples for biofilm cells and planktonic cells were withdrawn at four diffierent fermentation phases. The gene expression pattern of biofilm cells were investigated relative to that of planktonic cells from the same phase. The experiment was carried out twice independently. Cotton fibrous matrix (60 g/L) was used as biofilm carrier.
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| Contributor(s) |
Liu D, Xu J, Wang Y, Chen Y, Ying H |
| Citation(s) |
26621081 |
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| Submission date |
May 14, 2015 |
| Last update date |
Dec 04, 2015 |
| Contact name |
Dong Liu |
| Organization name |
Nanjing Tech University
|
| Department |
National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering
|
| Lab |
Prof. Hanjie Ying
|
| Street address |
No. 30, Puzhu South Road
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
211816 |
| Country |
China |
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| Platforms (1) |
| GPL19020 |
Agilent-053869 Clostridium acetobutylicum DSM 1731 and Arthrobacte sp. CGMCC 3584 8X15K array [Probe Name Version] |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE68901 |
Transcriptomic analysis of Clostridium acetobutylicum biofilm cells adsorbed onto fibrous matrix in biobutanol production |
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| Relations |
| BioProject |
PRJNA283991 |
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