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| Status |
Public on Feb 29, 2016 |
| Title |
Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow (I) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Human bone marrow mesenchymal stem cells (hBM-MSCs) hold promise for treating diseases for which currently no therapeutic options exist. High quantities of MSCs are needed, requiring extensive cell expansion in long-term culture. However, the fundamental biological properties of MSCs can be altered by culture conditions. In this study, hBM-MSCs were isolated from residual human bone marrow (hBM) material and expanded to clinically relevant numbers at passage 3-4. The cells had normal morphology, proliferation rate, immunophenotype and karyotype. Despite the chromosomal stability, after additional three passages (P6-P7) hBM-MSCs entered senescence state, confirmed by SA-β-galactosidase staining and paralleling the slower proliferation, altered morphology and imunophenotype. qRT-PCR array profiling revealed 3 out of 86 genes significantly (p <0.05) differentially (≥2 fold) expressed in the late passage cells compared to the early passage cells.
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| Overall design |
qPCR gene expression profiling. hBM-MSCs from 3 (1 male and 2 females) adult donors (age 30±7.21 years) were used. Each sample was tested in technical duplicate. 6 samples of early passage (P3-P4) hBM-MSCs were grouped to CONTROL group and 6 samples of late passage (P6-P7) hBM-MSCs were grouped to TEST group. The data were analyzed using web-based RT2 Profiler PCR Array Data Analysis v3.5 software (Qiagen)
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| Contributor(s) |
Kundrotas G, Gasperskaja E, Slapsyte G, Gudleviciene Z, Krasko J, Strumbryte A, Liudkeviciene R |
| Citation(s) |
26910916 |
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| Submission date |
May 15, 2015 |
| Last update date |
Feb 29, 2016 |
| Contact name |
Gabrielis Kundrotas |
| E-mail(s) |
gabrielis.kundrotas@nvi.lt
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| Organization name |
National Cancer Institute
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| Street address |
Santariškių str. 1
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| City |
Vilnius |
| ZIP/Postal code |
LT-08660 |
| Country |
Lithuania |
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| Platforms (1) |
| GPL20203 |
SABiosciences/Qiagen Human Mesenchymal Stem Cell RT² Profiler PCR Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE68933 |
Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow |
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| Relations |
| BioProject |
PRJNA284118 |
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