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| Status |
Public on Jan 01, 2016 |
| Title |
Gene expression data from mouse tracheal epithelial cells isolated before and 48hrs after SO2 injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The conducting airway epithelium of the rodent and human lung is made up of about equal proportions of ciliated and secretory cells. In addition, in regions where the epithelium is pseudostratfied, ~30% of the epithelium consists of undifferentiated basal cells (BCs). Evidence suggests that these BCs are multipotent stem cells that can self renew over the long term and give rise to both ciliated and secretory lineages. The goal of this project is to identify cellular and molecular mechanisms by which the basal cells normally maintain the epithelium and repair it after injury.
We used Affymetrix microarray analysis to compare transcripts in tracheal epithelium before and after SO2 injury.
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| Overall design |
Mice tracheal epithelium and mesenchyme were separate for RNA extraction before and 48hrs after SO2 injury. Cells from tracheas of 4 male C57Bl/6 mice were pooled for each biological experiment. The experiments were repeated three times for triplicate samples.
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| Contributor(s) |
Gao X, Hogan B |
| Citation(s) |
26811382 |
| |
| Submission date |
May 19, 2015 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Xia Gao |
| E-mail(s) |
xia.gao@dm.duke.edu
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| Organization name |
Duke University
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| Street address |
353 Nanaline Duke Bldg. Research Dr.
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE69058 |
Gene expression data from mouse tracheal cells before and 48hrs after SO2 injury |
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| Relations |
| BioProject |
PRJNA284411 |
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