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Series GSE6906 Query DataSets for GSE6906
Status Public on Jun 24, 2007
Title Rhythmic growth explained by coincidence between internal and external cues
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Plant hypocotyls elongate in response to darkness. The response to darkness is gated by the circadian clock, such that wild-type plants (Col) only respond to darkness with growth once every 24 hours, whereas arrhythmic lines, such as CCA1-34, will respond to darkness with growth at any time of day. The experiment here was designed to find genes whose expression was correlated with growth. It should also pick up other genes that are gated by the circadian clock or that are direct targets of CCA1.
Keywords: time course
Overall design Two genotypes were uses, wild-type (Col) and the arrhythmic CCA1-34 line that overexpresses the CCA1 protein. Plants were entrained in short day conditions for four days and then subjected light/dark pulses consisting of 160 minutes of light and 320 minutes of dark. We collected samples 120 minutes into the first and last dark periods of the day, corresponding to 280 and 1240 minutes after dawn. At the 280 minute time point Col plants are growing in response to darkness. At the 1240 minute time point Col plants are not growing. CCA1-34 plants are growing at both time points. The collection regime was continued for three days for a total of three temporal replicates for each time point/genotype combination.
Contributor(s) Nozue K, Covington MF, Harmer SL, Maloof JN
Citation(s) 17589502
Submission date Jan 30, 2007
Last update date Aug 28, 2018
Contact name Julin N Maloof
Phone (530) 752-8077
Fax (530) 752-5410
Organization name University of California, Davis
Department Plant Biology
Street address One Shields Ave
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM158680 Col_dark_early_rep1
GSM158681 Col_dark_late_rep1
GSM159273 Col_dark_early_rep2
Affiliated with GSE69995
BioProject PRJNA98157

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Supplementary file Size Download File type/resource
GSE6906_RAW.tar 24.6 Mb (http)(custom) TAR (of CEL, TXT)

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