|
| Status |
Public on Jun 15, 2016 |
| Title |
Crohn's disease risk variant in GPR65 alters lysosomal function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using an siRNA screen we identify a role for GPR65 in the defense against intracellular pathogens. Epithelial cells and macrophages lacking GPR65 exhibited impaired clearance of intracellular bacteria as well as an accumulation of aberrant phagosomes and lysosomes. Transcriptional profiling revealed changes in genes associated with lysosomal function.
|
| |
|
| Overall design |
Bone marrow-derived macrophages from WT or Gpr65-/- mice were harvested for RNA analysis.
|
| |
|
| Contributor(s) |
Lassen K, Goel G, Xavier RJ |
| Citation(s) |
27287411 |
| Submission date |
Jun 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ramnik J Xavier |
| E-mail(s) |
xavier@molbio.mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA285503 |
| SRA |
SRP058951 |
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