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| Status |
Public on Apr 14, 2016 |
| Title |
Whole-genome microarray analysis of human skin fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Connective tissue growthfactor (CTGF/CCN2) is a matricellular protein induced by transforming growth factor-beta (TGF-beta) and intimately involved with tissue repair and overexpressed in various fibrotic conditions. We have previously shown that keratinocytes in vitro downregulate TGF-beta induced expression of CTGF in fibroblasts by an interleukin-1 alfa (IL-1alfa) dependent mechanism. With this study we want to get a better overall perspective who fibroblasts respond on TGF-beta and in a TGF-Beta stinulated system, what effect addition of IL-1 alfa have. This to understand the expression of CTGF in human fibroblasts which in turn have implications for the pathogenesis of fibrotic conditions involving the skin.
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| Overall design |
Microarray was performed on human skin fibroblast RNA from one patient, Fibroblasts were seeded in vitro as a control (Control, C), TGF-beta were added to fibroblasts and incubated for 16 h (addition of TGF-beta, T) and both IL-1alfa and TGF-beta were added to fibroblasts for 16 h (addition of IL-1alfa and TGF-beta, IT). Each condition (C,T and IT) were done in three replicates.
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| Contributor(s) |
Koskela von Sydow A, Janbaz C, Kardeby CK, Repsilber D, Ivarsson M |
| Citation(s) |
26629874 |
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| Submission date |
Jun 02, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Anita Koskela von Sydow |
| Organization name |
Örebro University
|
| Street address |
Södra Grev Rosengatan 32
|
| City |
Örebro |
| ZIP/Postal code |
70362 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA285525 |
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