|
| Status |
Public on Apr 27, 2007 |
| Title |
Spermatozoal RNA Profiles (HumanRef-8 BeadChip) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Normal human spermatogenesis concludes with the formation of large numbers of morphologically well developed spermatozoa. While transcriptionally quiescent these cells carry an RNA payload that reflects the final spermiogenic phase of transcription. We report here the spermatozoal transcript profiles characteristic of normally fertile individuals and infertile males suffering from a consistent and severe teratozoospermia in which under 4% of spermatozoa are morphologically normal. RNA was extracted from the purified sperm cells of ejaculate and hybridized to Illumina WG ref8 Microarrays.
Keywords: disease state analysis
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| |
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| Overall design |
Spermatozoal RNAs were prepared from the semen samples of 10 individuals. An asymmetric dual block design was adopted with biological replicates in both blocks. 4 semen samples were assessed from normally fertile males. 6 semen samples were assessed from infertile individuals with a severe and consistent teratozoospermia (predominantly DFS).
|
| Web link |
http://compbio.med.wayne.edu
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| |
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| Contributor(s) |
Platts AE, Dix DJ, Chemes HE, Thompson KE, Goodrich R, Rockett JC, Rawe VY, Quintana S, Diamond MP, Strader LF, Krawetz SA |
| Citation(s) |
17327269 |
| Submission date |
Feb 06, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Stephen A Krawetz |
| E-mail(s) |
steve@compbio.med.wayne.edu
|
| Organization name |
Wayne State University
|
| Street address |
275 East Hancock, CS Mott Center
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48207 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2700 |
Sentrix HumanRef-8 Expression BeadChip |
|
| Samples (10)
|
| GSM160635 |
Teratozoospermic individual: Sample 23A |
| GSM160636 |
Teratozoospermic individual: Sample 23B |
| GSM160637 |
Teratozoospermic individual: Sample 23C |
| GSM160638 |
Teratozoospermic individual: Sample 23D |
| GSM160639 |
Teratozoospermic individual: Sample 23E |
| GSM160640 |
Teratozoospermic individual: Sample 23F |
| GSM160641 |
Normospermic individual: Sample 23G |
| GSM160642 |
Normospermic individual: Sample 23H |
| GSM160643 |
Normospermic individual: Sample 19B |
| GSM160644 |
Normospermic individual: Sample 19C |
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| This SubSeries is part of SuperSeries: |
| GSE6969 |
Spermatozoal RNA Profiles from Normally Fertile and Heterogeneously Teratozoospermic Subjects |
|
| Relations |
| BioProject |
PRJNA104125 |
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