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Series GSE69904 Query DataSets for GSE69904
Status Public on Jan 01, 2019
Title Arginine methylation regulates widespread alternative polyadenylation
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Alternative polyadenylation (APA) has recently been recognized as a universal mechanism for gene regulation, but it remains unclear how APA is controlled. Here we report that the Arabidopsis thaliana Protein Arginine Methyltransferase 10 (AtPRMT10) regulates global APA with its protein partner HLP1, a conserved hnRNP A/B protein. HLP1 binds preferentially to A-rich and U-rich cis-elements around polyadenylation sites, thereby linking AtPRMT10 to the control of APA through protein-protein interactions. AtPRMT10 mutations cause significant proximal-to-distal poly(A) site shifts largely overlapping with those in hlp1-1 mutants. Proximal polyadenylation is maintained by AtPRMT10-directed methylation and is mediated in part by methylation of HLP1 at specific arginine residues. Our findings demonstrate that arginine methylation of an RNA-binding protein adds a novel layer of regulation to widespread alternative polyadenylation.
Overall design Investigate the role of PRMT10 in alternative polyadenylation.
Contributor(s) Cao X
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Submission date Jun 15, 2015
Last update date May 15, 2019
Contact name xiaofeng cao
Phone 86-10-64869203
Organization name Institute of Genetics and Developmental Biology
Department State Key Laboratory of Plant Genomics
Street address West Lincui Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
Platforms (2)
GPL9302 Illumina Genome Analyzer II (Arabidopsis thaliana)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (11)
GSM954719 RNA-seq_prmt10
GSM1074237 PAS-seq_E152Q_mutant_rep1
GSM1074238 PAS-seq_E152Q_mutant_rep2
BioProject PRJNA287063
SRA SRP059933

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Supplementary file Size Download File type/resource
GSE69904_RAW.tar 116.8 Mb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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