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| Status |
Public on Mar 08, 2016 |
| Title |
Whole-transcriptome analysis of Staphylococcus aureus under laboratory and infection-mimicking conditions |
| Platform organism |
Staphylococcus aureus subsp. aureus NCTC 8325 |
| Sample organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
We report the condition-dependent transcriptome of S. aureus HG001, a derivative of strain NCTC 8325, by strand-specific tiling array hybridizations. More than 40 experimental conditions were investigated ranging from optimal in vitro growth to interaction with host cells. Analyses included the systematic mapping of transcription units, annotation of non-coding RNAs, the classification of promoters according to their dependency on SigA and SigB, and the prediction of potentially new transcription factor target sites. Antisense RNAs being of particular interest because of the small number of alternative sigma factors used by S. aureus were found to be relatively rare, overlapping only 6% of the annotated coding genes.
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| Overall design |
RNA samples prepared from S. aureus HG001 grown in shake flask or cell culture infection experiments were reverse transcribed, labeled and hybridized to tiling arrays.
Hybridizations were performed in triplicate using RNA isolated from independent cultures.
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| Contributor(s) |
Mäder U, Nicolas P, Depke M, Pané-Farré J, van der Kooi-Pol M, Reilman E, Hiron A |
| Citation(s) |
27035918 |
| |
| Submission date |
Jun 19, 2015 |
| Last update date |
Jun 07, 2016 |
| Contact name |
Pierre Nicolas |
| E-mail(s) |
pierre.nicolas@inrae.fr
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| Phone |
+33-1-3465-2894
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| Organization name |
INRAE - Université Paris-Saclay
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| Lab |
MaIAGE
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| Street address |
INRAE - Domaine de Vilvert
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| City |
Jouy-en-Josas |
| ZIP/Postal code |
F-78350 |
| Country |
France |
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| Platforms (1) |
| GPL20586 |
BaSysBio Staphylococcus aureus T1 385K array 080604_SA_JH_Tiling (gene level) |
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| Samples (156)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA287531 |
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