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Series GSE70228 Query DataSets for GSE70228
Status Public on Aug 22, 2017
Title Genomics insights into high pressure adaptation in a Thermococcus sp. from the world’s deepest hydrothermal vents
Organism Thermococcus
Experiment type Expression profiling by high throughput sequencing
Summary The present study describes the isolation of a Thermococcus sp. strain 175 from the world‘s deepest hydrothermal vent sites known thus far – The Mid-Cayman Rise.consisting of two hydrothermal venting systems Bee Bee (or Piccard), at 4950m depth and Von Damm (or Walsh) at 2300m The strain is capable of growth over 0.1MPa (atm. Pressure) to 120MPa, the widest known range of pressure dependent growth. The study further explores piezophilic adaptation using comparative genomic tools. Insights into the transcriptome of this strain providers the first look into the transcriptional machinery of peizophilic Thermococci.
 
Overall design RNA was extracted from cells grown at HighPressure (three replicates HP1,HP2,HP3) and at atmospheric pressure (reference also in triplicate Atm1,Atm2.Atm3) and sequenced.
 
Contributor(s) Narasingarao P, Bartlett DH, Latif H, Zengler K
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 24, 2015
Last update date May 15, 2019
Contact name Priya Narasingarao
Organization name Scripps Institution of Oceanography
Street address 8750 Biological Grade
City La Jolla
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL20620 Illumina MiSeq (Thermococcus)
Samples (6)
GSM1721015 ThermococcusHP1
GSM1721016 ThermococcusHP2
GSM1721017 ThermococcusHP3
Relations
BioProject PRJNA287920
SRA SRP059836

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70228_ThermococcusIsoforms_exp.diff.xlsx 312.0 Kb (ftp)(http) XLSX
GSE70228_Thermococcus_175_2545824513.genes.fna.gz 690.4 Kb (ftp)(http) FNA
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Raw data are available in SRA
Processed data are available on Series record

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