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| Status |
Public on Aug 31, 2015 |
| Title |
Identification of differentially expressed mRNAs in HCSC cell lines compared with HCC cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hep3B and Huh7 are two types of human hepatoma cell lines (HCC). In our laboratory, we cultured their stem-like cancer cells (HCSCs), Hep3B-C and Huh7-C. And we have demonstrated that these cells had enhanced stem cell properties, drug resistance, properties of EMT, and stronger tumor-initiating capabilities. To explore functionally crucial mRNAs in HCSCs, 2 samples of HCSCs and 2 samples of HCCs were sequenced by the Illumina Genome Analyzer II. Through differential expression analysis, we finally identified 115 up- and 402 down-regulated miRNAs which were consistently up- and down-regulated in two stem cells compared to the cancer cells.
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| Overall design |
Expression analysis using total RNAs extracted from 2 HCSC cell lines (Hep3B-C and Huh7-C), and 2 HCC cell lines (Hep3B and Huh7).
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| Contributor(s) |
Li J, Yan Z |
| Citation(s) |
26259570 |
| |
| Submission date |
Jul 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Min Ding |
| E-mail(s) |
dingmin0326@hotmail.com
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| Organization name |
Eastern Hepatobiliary Surgery Hospital
|
| Street address |
Changhai Road
|
| City |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA288962 |
| SRA |
SRP060359 |
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