|
| Status |
Public on Dec 22, 2015 |
| Title |
Genome-wide maps of KDM5 binding in drosophila adults |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine which genes affected by loss of KDM5 in adults were direct targets, we carried out KDM5 ChIP-seq analyses. To valide this data, we utilized a previously generated fly strain in which the sole source of KDM5 is from a transgene expressing an HA tagged form of KDM5 expressed under the control of its endogenous promoter. Comparing genome-wide gene expression and KDM5 binding analyses in Drosophila adults, we demonstrate the primary function of KDM5 in adults is to activate gene expression KDM5. To investigate the link between KDM5 and H3K4me3, we carried out anti-H3K4me3 ChIP-seq from wildtype adults . Genome-wide, KDM5 and H3K4me3 peaks showed a similar distribution, with both peaking at the transcription start site (TSS) showed a striking overlap with the presence of H3K4me3.
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| |
|
| Overall design |
Examination of KDM5 binding and histone H3K4me3 modifications in drosophila adults
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| |
|
| Contributor(s) |
Liu X, Secombe J |
| Citation(s) |
26673323 |
| |
| Submission date |
Jul 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Julie Secombe |
| E-mail(s) |
julie.secombe@einstein.yu.edu
|
| Organization name |
Albert einstein college of medicne
|
| Department |
Genetics
|
| Lab |
Julie secombe
|
| Street address |
1300 Morris park ave. Ullmann 809
|
| City |
Bronx |
| State/province |
United states |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE70591 |
KDM5 occupancy and expression profiling of kdm5 mutant flies and wt flies under normal condition and oxidative stress |
|
| Relations |
| BioProject |
PRJNA289118 |
| SRA |
SRP060443 |
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