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| Status |
Public on Dec 22, 2015 |
| Title |
RNA-seq analyses of kdm5 mutant flies and wt flies under normal condition and oxidative stress |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Comparing the transcriptome of wildtype and kdm5 mutant flies in normal conditions revealed a total of 4787 genes that were significantly downregulated and thus require KDM5 for their activation, and 3269 upregulated genes that are normally repressed by KDM5 (p<0.05, FDR <0.05). Because kdm5 mutants are sensitive to the oxidizer paraquat, we also carried out RNA-seq from wildtype and kdm5 mutant adults in oxidative stress conditions. Paraquat treatment of wildtype flies lead to the upregulation of 2481, and downregulation of 3103 genes
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| |
|
| Overall design |
adult mRNA profiles of 1-3-days old wild type (WT) and kdm5 mutant under normal condition and oxitative stress were generated by deep sequencing, using Illumina HisSeq 2000.
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| |
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| Contributor(s) |
Liu X, Secombe J |
| Citation(s) |
26673323 |
| |
| Submission date |
Jul 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Julie Secombe |
| E-mail(s) |
julie.secombe@einstein.yu.edu
|
| Organization name |
Albert einstein college of medicne
|
| Department |
Genetics
|
| Lab |
Julie secombe
|
| Street address |
1300 Morris park ave. Ullmann 809
|
| City |
Bronx |
| State/province |
United states |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE70591 |
KDM5 occupancy and expression profiling of kdm5 mutant flies and wt flies under normal condition and oxidative stress |
|
| Relations |
| BioProject |
PRJNA289117 |
| SRA |
SRP060444 |
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