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| Status |
Public on Jan 07, 2020 |
| Title |
Next generation sequencing allows identification of quantitave differential gene expression between four clinical strains of methicilin resistant Staphylococcus aureus |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Staphylococcus aureus can cause a broad spectrum of diseases that vary widely in clinical presentation and disease severity[121]. Methicillin-Resistant S. aureus (MRSA) strains first described in the 1960’s[122] were hospital acquired (HA MRSA), however in the 1990’s, community-associated MRSA strains (CA MRSA) were identified and are considered to be more virulent[16]. Therapeutics and management of MRSA focuses on novel antibacterials and vaccines targeting virulence factors. To date no clinical trials for vaccines have succeeded[123] due to the poor understanding of the pathogenic mechanisms exhibited by S.aureus.We investigated the differential gene expression of four clinical MRSA strains in vitro, belonging to HA and CA MRSA, at the stationary and exponential growth phases, using RNA-seq on the Ion torrent next generation sequencing platform. This study reveals the high diversity of virulence trait expression among MRSA strains within strains as well as between different growth phases, and also suggests potential factors other than PVL that contributes to enhanced virulence in CA MRSA
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| Overall design |
mRNA profiles were generated for four clinical strains of methicilin resistant staphylococcus aureus at their stationary growth phase, in duplicates and in exponential phase in triplicates, using RNA-seq in ion torrent platform. Data analysis performed with CLCBio Genomic Workbench, with two reference sequences, USA300_FPR3757 (NCBI RefSeq accession numbers NC_007793.1, NC_007790.1, NC_007791.1, NC_007792.1) and N315 strains (NCBI RefSeq accession numbers NC_002745.2, NC_003140.1)
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| Contributor(s) |
Tennakoon IU, Hyugens F |
| Citation missing |
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| Submission date |
Jul 08, 2015 |
| Last update date |
Jan 07, 2020 |
| Contact name |
Imalka U P K Tennakoon |
| E-mail(s) |
imalka123@yahoo.com
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| Phone |
+61420298510
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| Organization name |
Queensland University of Technology
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| Department |
Institute of Health and Biomedical Innovation
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| Street address |
60,Musk Avenue,Kelvingrove
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| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4059 |
| Country |
Australia |
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| Platforms (1) |
| GPL20673 |
Ion Torrent Proton (Staphylococcus aureus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA289253 |
| SRA |
SRP060528 |
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