|
| Status |
Public on Sep 11, 2015 |
| Title |
Enhancer divergence and cis-regulatory evolution in the human and chimpanzee neural crest |
| Organisms |
Pan troglodytes; Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Here we derive human and chimpanzee cranial neural crest cells (CNCCs) and profile histone modifications, transcription factors, chromatin accessibility and gene expression to systematically and quantitatively annotate evolutionary divergence of craniofacial cis-regulatory landscapes.
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| Overall design |
Histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), chromatin modifiers (p300), transcription factors (NR2F1, TFAP2A), chromatin accessibility (ATACseq) and gene expression (RNAseq) were assayed in CNCCs derived from iPSCs/ESCs from 2 chimpanzee and 3 human individuals.
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| |
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| Contributor(s) |
Prescott S, Wysocka J, Swigut T |
| Citation(s) |
26365491 |
| |
| Submission date |
Jul 10, 2015 |
| Last update date |
Feb 13, 2020 |
| Contact name |
Tomek Swigut |
| E-mail(s) |
swigut@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Chemical and Systems Biology
|
| Lab |
Wysocka
|
| Street address |
265 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (4)
|
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13802 |
Illumina Genome Analyzer IIx (Pan troglodytes) |
| GPL16809 |
Illumina HiSeq 2000 (Pan troglodytes) |
|
| Samples (64)
|
|
| Relations |
| BioProject |
PRJNA289483 |
| SRA |
SRP060650 |
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