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| Status |
Public on Jul 14, 2015 |
| Title |
Expression analysis of draculin (drl) expressing cells in embryonic zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
drl expression initiates during gastrulation and condenses as a band of cells at the prospective lateral embryo margin. In late epiboly, drl:EGFP is detectable as a band of scattered EGFP-fluorescent cells; after gastrulation, drl:EGFP-positive cells coalesce at the embryo margin that then in somitogenesis break down into the anterior and posterior lateral plate with subsequent cell migrations that form the posterior vascular/hematopoietic stripes and the anterior cardiovascular and myeloid precursors.
Data is as published in Mosimann & Panakova et al (Nature Communications, 2015)
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| Overall design |
The same pool of homozygous drl:EGFP transgenic zebrafish were mass-mated 3 times and embryos collected at 2ss. Embryos were de-chorionated, homogenized, and sorted, then subject to mRNA isolation and expression analysis.
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| Contributor(s) |
Zon L, Mosimann C |
| Citation(s) |
26306682 |
| |
| Submission date |
Jul 14, 2015 |
| Last update date |
Oct 13, 2015 |
| Contact name |
Gabriel Musso |
| E-mail(s) |
gabe.musso@gmail.com
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| Organization name |
Harvard Medical School
|
| Department |
Medicine
|
| Lab |
MacRae
|
| Street address |
75 Francis St, Thorn 1123
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL16933 |
[ZebGene-1_0-st] Zebrafish Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA289756 |
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