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Series GSE7107

Query DataSets for GSE7107

Status

Public on Feb 23, 2007

Title

An expression atlas of rice mRNAs and small RNAs

Organism

Oryza sativa

Experiment type

Expression profiling by MPSS

Summary

Identification of all expressed transcripts in a sequenced complex genome is technically challenging, but essential for systems biology and genome analysis. We used the transcriptional profiling technology called ‘massively parallel signature sequencing’ (MPSS) to develop a comprehensive expression atlas of rice (Oryza sativa cv Nipponbare). A total of 46,971,553 mRNA transcripts from 22 libraries, and 2,953,855 small RNAs from three libraries were sequenced. The data demonstrated widespread transcription throughout the genome, including expression for up to 25,500 annotated genes and antisense expression for nearly 9,000 annotated genes. An additional set of ~15,000 mRNA signatures mapped to unannotated genomic regions. The majority of the small RNA data represented lower abundance small-interfering RNAs (siRNAs) that match repetitive sequences, intergenic regions, and genes. Among these, numerous clusters of highly-regulated small RNAs were readily observed. We developed a genome browser (http://mpss.udel.edu/rice) for public access to the transcriptional profiling data for this important crop plant.
Keywords: MPSS, mRNA, small RNA, transcriptome, rice

Overall design

MPSS was performed to sequence small RNAs that derived from 16 untreated and 6 abiotic-treated diverse tissue libraries. 18 mRNA MPSS libraries and 3 small RNA libraries were submitted. We have total of 22 libraries (16 untreated and 6 abiotic-treated) mRNA libraries in our database, but it includes 4 mature leaf biological replicates and 2 mature root biological replicates. In order to reduce the redundancy, we created combined sets (NL4 and NR2) and submitted those data.

The method for the MPSS sequencing of mRNAs is described in Brenner et al. (Nat Biotechnol. 2000 18:630).

Contributor(s)

Meyers BC

Citation(s)

17351617

Submission date

Feb 22, 2007

Last update date

Mar 16, 2012

Contact name

Blake C. Meyers

E-mail(s)

bmeyers@danforthcenter.org