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Series GSE71266 Query DataSets for GSE71266
Status Public on Jul 24, 2015
Title Comparison of expression profiles for Agrobacterium tumefaciens wild-type strain C58 grown with or without manganese
Organism Agrobacterium tumefaciens
Experiment type Expression profiling by array
Summary The plant pathogen Agrobacterium tumefaciens attaches to and forms biofilms on both biotic and abiotic surfaces. The transition between free-living, planktonic A. tumefaciens and multicellular biofilms is regulated by several well-defined environmental and nutritional inputs, including pH, oxygen tension, and phosphate concentration. In many bacterial species limiting iron levels inhibit attachment and biofilm formation. In several systems intracellular levels of the redox-active manganous (Mn2+) and ferrous (Fe2+) ions are interrelated and have tight corresponding regulation with respect to one another. We show that limiting manganese concentrations elicit similar growth and biofilm phenotypes to those seen under iron-limiting conditions. Microarray analysis comparing gene expression in manganese-replete versus manganese-limiting conditions identified a small number of differentially regulated transcripts. These results indicate that the redox-active manganous and ferrous ions are required for wild-type levels of growth and biofilm formation, and that the manganese-dependent response is primarily post-transcriptional and complementary to, but not redundant with, the iron-dependent response.
Overall design Four biological replicates, independent RNA preparations, two dye swaps.
Contributor(s) Heindl JE, Hibbing ME, Xu J, Fuqua C
Citation(s) 26712936
Submission date Jul 23, 2015
Last update date Apr 05, 2016
Contact name Jason Eugene Heindl
Phone 215-596-7423
Organization name Indiana University
Department Biology
Lab Clay Fuqua
Street address 1001 E. 3rd St., Jordan Hall 142
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL18993 Agilent-012657 Agrobacterium tumefaciens 11K array
Samples (4)
GSM1831639 A. tumefaciens_Manganese_Rep1
GSM1831640 A. tumefaciens_Manganese_Rep2
GSM1831641 A. tumefaciens_Manganese_Rep3
BioProject PRJNA290725

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71266_LimmaOutputDescription.pdf 60.3 Kb (ftp)(http) PDF 472.3 Kb (ftp)(http) XLSX
GSE71266_RAW.tar 3.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
Processed data are available on Series record

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