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| Status |
Public on Dec 18, 2016 |
| Title |
Cooperation between H3K27me3 and non-CG methylation in epigenetic regulation of genes in rice. |
| Organism |
Oryza sativa Japonica Group |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
H3K27me3 is a repressive chromatin mark of genes and is catalyzed by homologs of Enhancer of zeste [E(z)], a component of Polycomb-repressive complex 2 (PRC2), while DNA methylation that occurs in CG and non-CG (CHG and CHH, where H is A, C or T) contexts is a hallmark of transposon silencing in plants. However, relationship between H3K27me3 and DNA methylation in gene repression remains unclear. In addition, the mechanism of PRC2 recruitment to specific genes is not known in plants. Here, we show that SDG711, a rice E(z) homolog, is required to maintain H3K27me3 of many developmental genes after shoot meristem to leaf transition and that many H3K27me3-marked developmental genes are also methylated at non-CG sites in the body regions. SDG711-binding and SDG711-mediated ectopic H3K27me3 also target genes methylated at non-CG sites. Conversely, mutation of OsDRM2, a major rice CHH methyltransferase, resulted in loss of SDG711-binding and H3K27me3 from many genes and their de-repression. Furthermore, we show that SDG711 physically interacts with OsDRM2 and a putative CHG methylation-binding protein. These results together suggest that the repression of many developmental genes may involve both DRM2-mediated non-CG methylation and PRC2-mediated H3K27me3 and that the two marks are not generally mutually exclusive but may cooperate in repression of developmentally regulated genes in rice.
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| Overall design |
Genome-wide mapping of H3K27me3 and/or DNA methylation in WT, SDG711 overexpression, and osdrm2 mutant. Meterials and sequencing for BS-seq of WT and SDG711 overexpression are harvested or done at the same time.
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| |
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| Contributor(s) |
Zhou S, Liu X, Zhou C, Zhou D |
| Citation(s) |
27535791 |
| |
| Submission date |
Aug 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
shaoli zhou |
| E-mail(s) |
zhoushaoli@mail.hzau.edu.cn
|
| Organization name |
Huazhong Agricultural University
|
| Department |
National Key Laboratory of Crop Genetic Improvement
|
| Street address |
shizishan street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
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| Platforms (2) |
| GPL13834 |
Illumina HiSeq 2000 (Oryza sativa Japonica Group) |
| GPL22250 |
Illumina HiSeq 3000 (Oryza sativa Japonica Group) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA291819 |
| SRA |
SRP061969 |
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