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| Status |
Public on Aug 05, 2007 |
| Title |
Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO) |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC.
Keywords: time course
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| Overall design |
Three wild-type T. thermophilus HB8 strains were pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.
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| Contributor(s) |
Nakagawa N, Kashihara A, Shinkai A, Yokoyama S, Kuramitsu S |
| Citation(s) |
17369302, 17905996 |
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| Submission date |
Mar 01, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
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| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL4902 |
Riken Thermus thermophilus HB8 4K mRNA array |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE7175 |
SuperSeries for the study of expression analysis of the T. thermophilus CRP (TTHA1437) |
| GSE8781 |
SuperSeries for the study of expression analysis of the T. thermophilus sigE (TTHB211) |
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| Relations |
| BioProject |
PRJNA104053 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7165_RAW.tar |
29.9 Mb |
(http)(custom) |
TAR (of CEL) |
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