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| Status |
Public on Jul 22, 2016 |
| Title |
Expression data from Human Ishikawa cells treated with Genistein |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study provides a comprehensive evaluation of changes in gene expression during treatment with Genistein in vitro.
Ishikawa cells were maintained and grown (to confluency or at a desired cell density between 500,000 - 1 million cells/mL) in DMEM/F-12 Medium (supplemented with 10% Fetal Bovine Serum + 1X penicillin/streptomycin) in xenoestrogen-free Corning plasticware. Cells were gently washed in warm PBS and transferred to phenol red-free DMEM/F-12 Medium (supplemented with 10% Charcoal-Stripped Fetal Bovine Serum + 1X penicillin/streptomycin) in Corning Cell Culture Cluster wells overnight and then challenged with 10pM (very low, vL), 1 nM (low, L), 10nM (high, H), and 1uM (very high, vH) levels of Genistein. Prior to collection, cells were washed in warm PBS, resuspended & briefly incubated in TRI-Reagent, & finally collected (in quintuplicate replicates) at time each specific time point: 8 hours, 24 hours, and 48 hours. Following RNA isolation, the best RNA yields for each quadruplicate set was selected for target preparation and GeneChip processing.
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| Overall design |
60 total samples: 5 Doses [Vehicle Control, Very Low (1 pM), Low (100 pM), High (1 nM), Very High (1 uM)]; 3 Timepoints [8 hour, 24 hour, 48 hour]; 4 Replicates each
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| Contributor(s) |
Tiesman JP |
| Citation(s) |
26865667 |
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| Submission date |
Aug 04, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jay Patrick Tiesman |
| Organization name |
Procter & Gamble
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| Department |
Global Biotechnology
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| Lab |
Mason Business Center
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| Street address |
PO BOX 8006
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| City |
MASON |
| State/province |
OH |
| ZIP/Postal code |
45040-8006 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA291928 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71717_RAW.tar |
329.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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