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| Status |
Public on Sep 24, 2015 |
| Title |
RNA-seq data of intestinal epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report that adhesion of microbes to intestinal epithelial cells is a critical cue for Th17 induction. SFB colonized in the intestine of mice can adhere to mouse small intestinal epithelial cells and induce intestinal Th17 cells. However, SFB colonized in rats cannot adhere to mouse intestinal epithelial cells and induce Th17 cells. Likewise, Citrobacter rodentium (WT) can adhere to mouse colonic epithelial cells and induce Th17 cells, but non-adherent mutant of C. rodentium (Δeae) cannot induce Th17 cells. To examine the influence of adherent bacteria on intestinal epithelial cells, we performed RNA seq. Germ free mice were orally inoculated with M-SFB or R-SFB and total RNA was isolated from small intestinal epithelial cells 1 week after inoculation. Alternatively, germ free mice were orally inoculated with C. rodentium WT or eae mutant and total RNA was isolated from colonic epithelial cells 5 days after inoculation.
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| Overall design |
The gene expression of small intestinal epithelial cells isolated from small intestine of germ free mice (2 mice), mice monocolonized with M-SFB (2 mice) or R-SFB (3 mice), and colon of germ free mice (3 mice), mice monocolonized C. rodentium WT (3 mice) or eae mutant (3 mice).
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| Contributor(s) |
Atarashi K, Tanoue T, Nagano Y, Umesaki Y, Honda K |
| Citation(s) |
26411289 |
| |
| Submission date |
Aug 05, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Koji Atarashi |
| E-mail(s) |
kojiatarashi@keio.jp
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| Organization name |
Keio University School of Medicine
|
| Department |
Dept of Microbiology and Immunology
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku-ku |
| State/province |
TOKYO |
| ZIP/Postal code |
1608582 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA291962 |
| SRA |
SRP062039 |
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